Fatty Acid Supplementation Reverses the Small Colony Variant Phenotype in Triclosan-Adapted Staphylococcus aureus: Genetic, Proteomic and Phenotypic Analyses
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چکیده
منابع مشابه
Phenotype microarray profiling of Staphylococcus aureus menD and hemB mutants with the small-colony-variant phenotype.
Standard biochemical tests have revealed that hemin and menadione auxotrophic Staphylococcus aureus small-colony variants (SCVs) exhibit multiple phenotypic changes. To provide a more complete analysis of the SCV phenotype, two genetically defined mutants with a stable SCV phenotype were comprehensively tested. These mutants, generated via mutations in menD or hemB that yielded menadione and he...
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variants (SCVs) !" sub-MIC triclosan (TLN) # $ %& ' ( !) TLN SCVs*"+,!" "+#. 2 $'(/0 %1, 4 $ . &' 2&34$56* SCVs (7,68!" %9: SCVs;. MIC*)< * 25+= ;',$ >DNase .? @ABCDEF/ G4H0IJKLM12 3NO * 4$ PQ R( 5S6TU7V:9!" =! TLN WDXYZ;R enoyl reductase enzyme (FabI) Ala95[ValU,\. Thr147[His A]^_(` /a68!" TLN SCVs. b 886c9:;,6U *? =;o@,!"A '...
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The in vitro bactericidal activities of vancomycin against Staphylococcus aureus hemB mutants displaying the small-colony-variant phenotype and their parental strains were evaluated. Vancomycin killing activities against hemB mutants were markedly attenuated, demonstrating approximately 50% less effect, a result which was well described by a Hill-type pharmacodynamic model.
متن کاملDaptomycin pharmacodynamics against Staphylococcus aureus hemB mutants displaying the small colony variant phenotype.
OBJECTIVES Staphylococcus aureus small colony variants (SCVs) are slow-growing morphological variants associated with persistent infections. While vancomycin activity has been shown to be attenuated against SCVs of S. aureus, few data exist regarding daptomycin. The objective was to evaluate the pharmacodynamics of daptomycin against defined S. aureus mutants displaying the SCV phenotype. MET...
متن کاملSmall-colony variants: a novel mechanism for triclosan resistance in methicillin-resistant Staphylococcus aureus.
OBJECTIVES A little-understood mode of antimicrobial resistance in Staphylococcus aureus is the evolution of a sub-population of small-colony variants (SCVs). SCVs are a cause of persistent and recurring infections refractory to antimicrobial chemotherapy. Following the inadvertent isolation of suspected SCVs growing in the presence of triclosan we set out to evaluate the formation of these col...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2018
ISSN: 2045-2322
DOI: 10.1038/s41598-018-21925-6